In this proposal we will test the hypothesis that the physiologic progenitor cell of mesodermal derivatives, the mesenchymal stem cell (MSC), is the cell of origin for a diverse population of sarcomas. We will isolate MSC based upon their functional activation of a marker gene (GFP) through Wnt/beta-catenin signal transduction. We will test a highly enriched MSC population for clonal self-renewal in vitro, and for tumor initiating capacity in vivo. The objective of this proposal is to create a xenogenic model of human sarcoma in the immune deficient Rag2 mouse for studies on the initiation/promotion of sarcoma.
Karl Sylvester, MD, Stanford University
Recipient of the: $25,000 Seth A. Bailey Memorial Research Award